{"product_id":"the-influence-of-hypoxia-on-gata-1-and-epo-expression-levels-in-developing-zebrafish-von-markus-holotta","title":"The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish","description":"\u003cp\u003eMaster's Thesis from the year 2007 in the subject Biology - Zoology, grade: 1,0, University of Innsbruck (Zoologie), language: English, abstract: The transcription factor GATA-1 is essential for the development of the erythroid cell lineage in vertebrates. In this article we introduce a method to easily determine the approximately development status of red blood cells and the progression of blood formation by intensity of fluorescence in GATA-1\/Ds-Red marked zebrafish.  We classified the blood cells on the basis of their fluorescence intensity in 5 intensity stages (IS) with the brightest in IS 1. The comparison with our erythropoietin (Epo) data showed a noticable correlation between GATA-1, Epo mRNA and EPO protein level. Between 2 and 3 dpf we observed a major increase in blood cell concentration to circa 1200 cells*nl-1, until 15 dpf this value decreased to about the half. The appearance of IS 1 cells correspond approximately to the peaks in Epo cRNA copies and the highest values in EPO protein emerged about 1 day later. Our data show that the blood cell concentration, Epo and Gata-1 expression in zebrafish larvae is subjected to large fluctuations in the first few days of development.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783638905718\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783638905718","offer_id":39439122628701,"sku":"9783638905718","price":17.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/a75ab31d-5a57-43a6-b3f0-941a06b517e0.jpg?v=1777441869","url":"https:\/\/shop.autorenwelt.de\/products\/the-influence-of-hypoxia-on-gata-1-and-epo-expression-levels-in-developing-zebrafish-von-markus-holotta","provider":"Autorenwelt Shop","version":"1.0","type":"link"}