{"product_id":"the-function-of-mrn-complex-during-wrn-facilitated-atm-activation-von-junhao-ma","title":"THE FUNCTION OF MRN COMPLEX DURING WRN FACILITATED ATM ACTIVATION","description":"\u003cp\u003eWRN (Werner) protein is a member of the RecQ family showing helicase and exonuclease activity. WRN protein may lose function upon mutation and causes Werner syndrome (WS) which is a cancer-prone and premature aging disease. ATM (Ataxia-Telangiectasia mutated) protein initiates a signaling pathway in response to DNA double strand breaks (DSBs). Genomic disorder ataxia-telangiectasia (A-T) is associated with defective ATM. WRN protein is involved in ATM pathway activation when cells are exposed to DSBs associated with replication fork collapse. Because the Mre11-Rad50-Nbs1 (MRN) complex, a sensor of DSBs, is known to interact with WRN and ATM, we investigated whether the MRN complex mediates the WRN-dependent ATM pathway activation. In this study, we employed short-hairpin RNA to generate WRN- and Nbs1-deficient U-2 OS (osteosarcoma) cells. Cells were treated with clastogens which induce collapsed replication forks, thus provided proof for whether WRN facilitates ATM activation via MRN complex. This study serves as a basis for future investigation on the correlation between ATM, MRN complex and WRN, which will help understand the mechanism of aging and cancer.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838353616\"\u003e\u003ch3\u003eTHE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838353616","offer_id":39499066376285,"sku":"9783838353616","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/281ce2b1-baae-44bc-99ae-2f0d9bf5de8c.jpg?v=1758875553","url":"https:\/\/shop.autorenwelt.de\/products\/the-function-of-mrn-complex-during-wrn-facilitated-atm-activation-von-junhao-ma","provider":"Autorenwelt Shop","version":"1.0","type":"link"}