{"product_id":"the-effect-of-mutating-the-pdz-domains-within-secreted-pdzd2-von-zee-man-wat","title":"The Effect of Mutating the PDZ domains within secreted PDZD2","description":"\u003cp\u003ePDZ domains are one of the most common protein-protein interaction domains in human.  PDZ domain containing 2 protein (PDZD2) contains 6 PDZ domains and is highly expressed in pancreatic beta cells.  It undergoes cleavage in the endoplasmic reticulum to produce a secreted form PDZD2 (sPDZD2) which retains the last two PDZ domains of PDZD2.  sPDZD2 is a candidate beta cell regulatory factor.  In current study, two mutated forms of sPDZD2 were generated with alterations in the amino acid sequence within the carboxylate-binding loop of one of the two PDZ domains, either at the PDZ5 or at the PDZ6 domain.  Current study revealed that both the PDZ5-mutated and PDZ6-mutated sPDZD2 proteins failed to exert the insulinotropic effect that the wildtype sPDZD2 had on INS-1E cells when added exogenously in the culture medium.  Both mutant proteins also failed to rescue the silencing action of siRNA on the insulinotropic effect of endogenous PDZD2 in INS-1E cells.  These results suggest that intact carboxylate-binding loops of both PDZ5 and PDZ6 domain are crucial for the insulinotropic effect of sPDZD2 exerting on INS-1E cells.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844385335\"\u003e\u003ch3\u003eon its insulinotropic action in INS-IE cells\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844385335","offer_id":39493573574749,"sku":"9783844385335","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/cf7aa829-64ef-409c-bfa8-16fb873eebb6.jpg?v=1773296920","url":"https:\/\/shop.autorenwelt.de\/products\/the-effect-of-mutating-the-pdz-domains-within-secreted-pdzd2-von-zee-man-wat","provider":"Autorenwelt Shop","version":"1.0","type":"link"}