{"product_id":"systems-metabolic-engineering-of-escherichia-coli-for-production-of-violacein-and-deoxyviolacein-von-andre-luis-rodrigues","title":"Systems metabolic engineering of Escherichia coli for production of violacein and deoxyviolacein","description":"\u003cp\u003eViolacein and deoxyviolacein are promising therapeutics against pathogenic bacteria and viruses as well as tumor cells. In the present work, systems-wide metabolic engineering was applied to Escherichia coli for heterologous production of these high-value products. First, a high performance liquid chromatography method for accurate separation and quantification of violacein and deoxyviolacein was developed. Afterwards, a basic producer, E. coli dVio-1, that expressed the vioABCE cluster from Chromobacterium violaceum under control of the araBAD promoter and induction by L-arabinose, was constructed. Targeted intracellular metabolite analysis then identified bottlenecks in pathways that supply tryptophan, the major product building block of the natural products of interest. This was used for systems-wide engineering of serine, chorismate and tryptophan biosynthesis and the non-oxidative pentose-phosphate pathway, followed by elimination of L-arabinose catabolism. Transferred to a glycerol-based fed-batch process, E. coli dVio-8 surpassed the gram scale and produced 1.6 g L-1 deoxyviolacein (\u0026gt; 99.5% purity). The created chassis of a high-flux tryptophan pathway was complemented by genomic integration of the vioD gene of Janthinobacterium lividum, which enabled exclusive production of violacein (710 mg L-1 with 99.8% purity). This demonstrates the potential of E. coli as a platform for production of tryptophan based therapeutics.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783954049608\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783954049608","offer_id":39463094845533,"sku":"9783954049608","price":28.5,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/cc7c89d9-3df3-4f89-ad0b-6bf2bdc793c5.jpg?v=1776496276","url":"https:\/\/shop.autorenwelt.de\/products\/systems-metabolic-engineering-of-escherichia-coli-for-production-of-violacein-and-deoxyviolacein-von-andre-luis-rodrigues","provider":"Autorenwelt Shop","version":"1.0","type":"link"}