{"product_id":"synthesis-of-tyrosinase-by-streptomyces-sp-von-raghavendra-h","title":"Synthesis of tyrosinase by streptomyces SP.","description":"\u003cp\u003e¿ In all 15 isolates of Streptomyces were screened for the synthesis of tyrosinase by both qualitative and quantitative methods in synthetic medium. ¿ Activity of tyrosinase, specific activity of tyrosinase, formation of melanin and protein content of tyrosinase were assessed to determine the catalytic power of tyrosinase. ¿ In a batch wise bioprocess, an isolate of Streptomyces DLS-8 has been found to be potential for the production of tyrosinase. ¿ Effect of pH and temperature on the production of tyrosinase were also examined. 300C temperature and pH 8.5 were proved to be optimum for the maximum production of tyrosinase. ¿ Results of the present study revealed that starch and citrulline were the important carbon and nitrogen sources respectively for the maximum production of tyrosinase. ¿ The partial isolation and purification of the tyrosinase was under taken by SDS-PAGE. The tyrosinase was determined to be about 45 KDa, comparing to BSA as standard marker.¿ Liquid chromatogram mass spectrum reveals the presence of tyrosine, L-Dopa and melanin based on their base peaks for their respective molecular weights indicating significantly the catalytic power of tyrosinase.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786207473359\"\u003e\u003ch3\u003eEco-friendly synthesis of tyrosinase using streptomyces SP.\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9786207473359","offer_id":48495998402885,"sku":"9786207473359","price":60.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/c67b3dc2-16ca-483d-91d2-30f36d503f5a.jpg?v=1759208613","url":"https:\/\/shop.autorenwelt.de\/products\/synthesis-of-tyrosinase-by-streptomyces-sp-von-raghavendra-h","provider":"Autorenwelt Shop","version":"1.0","type":"link"}