{"product_id":"synthesis-and-enzymatic-testing-of-reversible-terminators-for-sbs-von-angelika-keller","title":"Synthesis and enzymatic testing of reversible terminators for SBS","description":"\u003cp\u003eBased on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3¿-modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3¿-modification) that the DNA-synthesis can continue (i. e. ¿reversible¿ termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3¿-modified nucleosides as key compounds for these terminators and the evaluation of suitable 3¿-modification cleavage conditions.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838113555\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783838113555","offer_id":39435654463581,"sku":"9783838113555","price":89.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/248322f3-458d-41b7-b8aa-9c07dee286aa.jpg?v=1777957272","url":"https:\/\/shop.autorenwelt.de\/products\/synthesis-and-enzymatic-testing-of-reversible-terminators-for-sbs-von-angelika-keller","provider":"Autorenwelt Shop","version":"1.0","type":"link"}