{"product_id":"spectroscopic-studies-of-structure-and-function-of-the-light-gated-cation-channel-channelrhodopsin-2-von-melanie-hey","title":"Spectroscopic Studies of Structure and Function of the Light-Gated Cation Channel Channelrhodopsin-2","description":"\u003cp\u003eRevealing structural and mechanistic details of Channelrhodopsin-2 (ChR2) is in the focus of current scientific research due to its unique ability to stimulate cell activity by light (optogenetics). Hence, ChR2 is a promising tool to revolutionize medical treatment. The aim of this work was the investigation of the light-activated mechanism of the retinylidene cation channel ChR2 on an atomistic level by means of vibrational spectroscopy.\u003c\/p\u003e\u003cp\u003eIn addition to successful expression and purification of ChR2, resonance Raman and FTIR spectroscopy elucidated the structure of the chromophore binding pocket as well as the gating mechanism triggered by a single hydrogen bond between two residues (¿DC gate¿). Therefore, FTIR difference spectroscopic results were correlated with time-resolved UV\/Vis spectroscopy. Flash photolysis allowed characterization of the time scale of proton release with subsequent uptake using an indicator dye.\u003c\/p\u003e\u003cp\u003eApplication and modification of advanced biophysical techniques such as surface-enhanced FTIR, single-molecule force spectroscopy and doubly vibrationally-enhanced four wave mixing set the basis to obtain even deeper insights into the structure and function of membrane proteins like ChR2.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783954046355\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783954046355","offer_id":39462937034845,"sku":"9783954046355","price":42.35,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/18ed0bb9-919c-4d74-a9ac-35a429b7b6f6.jpg?v=1780376513","url":"https:\/\/shop.autorenwelt.de\/products\/spectroscopic-studies-of-structure-and-function-of-the-light-gated-cation-channel-channelrhodopsin-2-von-melanie-hey","provider":"Autorenwelt Shop","version":"1.0","type":"link"}