{"product_id":"rna-interference-in-human-embryonal-carcinoma-and-embryonic-stem-cells-von-maryam-m-matin-und-peter-w-andrews","title":"RNA INTERFERENCE IN HUMAN EMBRYONAL CARCINOMA AND EMBRYONIC STEM CELLS","description":"\u003cp\u003eHuman embryonal carcinoma (hEC) cells are the stem  cells of teratocarcinomas. Human embryonic stem  (hES) cells are pluripotent cells derived from  inner cell mass of human embryos at blastocyst  stage, and can be induced to differentiate into all  three embryonic germ layers. hES cells offer  the opportunity for in vitro production of multiple  cell types for use in regenerative medicine. A key  to unlocking this potential is development of  methods for controlling gene expression and,  consequently, cell differentiation. One tool that  might be exploited is RNA interference (RNAi) to  manipulate specific signalling pathways in a  transient manner and so influence the selection of  specific pathways of differentiation by a stem  cell.  To explore this possibility we used RNAi to  determine whether the transcription factor, OCT4,  is  required to maintain the undifferentiated state of  hEC, as well as hES cells and whether forced  knockdown of OCT4 expression results in  differentiation towards trophectoderm. This opens  up  the possibility of targeting genes known to play a  role in pluripotency or malignancy, using RNAi  technologies, in cancer therapy.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838379111\"\u003e\u003ch3\u003esiRNA mediated knockdown OF GFP, ¿2-microglobulin and OCT4\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838379111","offer_id":39499028627549,"sku":"9783838379111","price":59.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/5aa4955c-578f-404f-8d4d-c2d218048b36.jpg?v=1759294149","url":"https:\/\/shop.autorenwelt.de\/products\/rna-interference-in-human-embryonal-carcinoma-and-embryonic-stem-cells-von-maryam-m-matin-und-peter-w-andrews","provider":"Autorenwelt Shop","version":"1.0","type":"link"}