{"product_id":"rna-dependent-selenocysteine-biosynthesis-in-eukaryotes-and-archaea-von-sotiria-palioura","title":"RNA-dependent Selenocysteine Biosynthesis in Eukaryotes and Archaea","description":"\u003cp\u003eSelenocysteine (Sec), the 21st genetically encoded amino acid, is the major metabolite of the micronutrient selenium. Sec is inserted into nascent proteins in response to a recoded UGA stop codon. The substrate for ribosomal protein synthesis is selenocysteinyl-tRNA. The mechanism of Sec-tRNA formation was known in bacteria, but remained poorly understood in archaea and eukaryotes. Herein, biochemical and genetic data provide evidence that Sec-tRNA biosynthesis requires the tRNA-dependent conversion of O-phosphoserine (Sep) to Sec in eukaryotes and archaea. In this two-step pathway O-phosphoseryl-tRNA kinase (PSTK) first converts seryl-tRNA to Sep-tRNA, which is then the obligatory precursor for a Sep-tRNA: Sec-tRNA synthase (SepSecS). SepSecS used to be annotated as Soluble Liver Antigen\/Liver Pancreas (SLA\/LP) and is thought to be the antigen for a form of autoimmune hepatitis. The crystal structure of human SepSecS complexed with its substrates sheds light to the vitamin B6-dependent mechanism of Sec-tRNA formation. This report is useful to biophysicists, biochemists, and evolutionary biologists as well as to anyone investigating the role of SepSecS in autoimmune hepatitis.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783639709032\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783639709032","offer_id":39482486816861,"sku":"9783639709032","price":38.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/4e2251ab-001a-4ecf-998f-f512b26e4d79.jpg?v=1773380389","url":"https:\/\/shop.autorenwelt.de\/products\/rna-dependent-selenocysteine-biosynthesis-in-eukaryotes-and-archaea-von-sotiria-palioura","provider":"Autorenwelt Shop","version":"1.0","type":"link"}