{"product_id":"purification-and-characterization-of-beta-amylase-from-bacillus-subtilis-isolated-from-fermented-african-locust-bean-von-temitope-abu","title":"Purification and Characterization of Beta-amylase","description":"\u003cp\u003e¿-amylase was purified and characterized from Bacillus subtilis isolated from fermented Parkia biglobosa seeds. Purification was achieved using ion exchange DEAE column and gel filtration (Sephadex G-200) chromatography. Effects of temperature; pH and production time on ¿-amylase production were investigated, while physicochemical characteristics of the purified enzyme were investigated. The optimum production of ¿-amylase was obtained at temperature, pH and time of 37oC, 7.0 and 24 h respectively. The results showed that purified ¿-amylase had more enzymatic activity than crude samples from Bacillus subtilis whereby the activity of crude enzyme was 3.21 mM\/min\/ml while the purified enzyme had an improved activity of 21.46 mM\/min\/ml. Optimum temperature and pH values of the purified amylase were found to be 50°C and pH 5.0, respectively. pH stability of the enzyme ranged from pH 4.0- 9.0. At pH 5.0 and 7.0 it retained 70% and 60% of its activity respectively after 5 h of incubation. Temperature stability ranged between 40oC and 70oC but most stable at 50oC retaining 64% of its activity after 1 h of incubation. The enzyme exhibited maximum activity on soluble starch and sucrose.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659684258\"\u003e\u003ch3\u003eFrom Bacillus Subtilis Isolated from Fermented African Locust Bean\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783659684258","offer_id":39431619412061,"sku":"9783659684258","price":39.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/ba708e5d-9cf6-45e3-83df-36139639e1a1.jpg?v=1773381562","url":"https:\/\/shop.autorenwelt.de\/products\/purification-and-characterization-of-beta-amylase-from-bacillus-subtilis-isolated-from-fermented-african-locust-bean-von-temitope-abu","provider":"Autorenwelt Shop","version":"1.0","type":"link"}