{"product_id":"production-of-radiolabeled-e-peptide-von-christine-sallum","title":"Production of Radiolabeled E-Peptide","description":"\u003cp\u003eEa4 peptide of rainbow trout pro-IGF-I has been shown to exert anti-cancer activities in human cancer cell lines.  Kou and Chen (2003) have shown that the trout Ea-4 peptide could bind specifically with a binding component on the membrane of neuroblastoma cells (SK-NF-1).  In order to characterize this binding component, a radio-labeled ligand with high specific activity will be required.  In this study, effort was made to label trout Ea4-peptide by phosphorylation by protein kinase A (PKA) with radioactive ATP.  Recombinant PKA4 peptide was prepared by adding 5 amino acid residues to the carboxyl-terminal of the trout Ea4-peptide.  Following partial purification, recombinant PKA4 peptide and Ea4-peptide were active in mitogenesis in mouse fibroblast cells and morphological differentiation in SK-N-SH neuroblastoma cells.  By protein kinase reaction, recombinant PKA4 peptide was labeled with 32P-ATP.  Both rtEa4 and PKA4 peptides were labeled with 32P using the PKA enzyme. PKA4 peptide was labeled at levels over 10 times the expected values.  Four serine phosphorylation sites and 3 threonine phosphorylation sites on the rtEa4 peptide may have contributed to the phosphorylation.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838374901\"\u003e\u003ch3\u003eBiologically Active Anti-Cancerous Peptide\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838374901","offer_id":39498946642013,"sku":"9783838374901","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/38c7e387-ffcb-4b1f-a5f0-31701e1a546d.jpg?v=1757655712","url":"https:\/\/shop.autorenwelt.de\/products\/production-of-radiolabeled-e-peptide-von-christine-sallum","provider":"Autorenwelt Shop","version":"1.0","type":"link"}