{"product_id":"production-of-labyrinthopeptin-a1-with-actinomadura-namibiensis-von-sebastian-tesche","title":"Production of labyrinthopeptin A1 with Actinomadura namibiensis","description":"\u003cp\u003eThe filamentous actinomycete Actinomadura namibiensis is the only known producer of labyrinthopeptins, a class of ribosomally synthesized and posttranslationally modified peptides (RiPPs) displaying highly attractive bioactive properties. In order to increase the labyrinthopeptin A1 productivity in shaking flask cultivations of A. namibiensis, a new cultivation method called salt-enhanced cultivation was used. Compared to the unsupplemented control, labyrinthopeptin A1 productivity was enhanced the most by addition of 50 mM (NH4)2SO4, reaching a 7-fold higher yield of 325 mg L-1 within 10 cultivation days. Salt-enhanced cultivation affected growth and product formation mechanisms, cell morphology characteristics and rheological characteristics of cultivation broth. An image analysis method was developed to quantify both the macro-morphology (pellet size and shape) and the micro-morphology (hyphal network structure) of the heterogeneous filamentous biomass in detail. Productivity-related morphological parameters were in particular the size and circularity of pellets and the degree of hyphal interweaving (hyphal network spacing). It was shown that the time-dependent change in morphology linked to the rheological properties of the cultivation broth.\u003c\/p\u003e\u003cp\u003eThe results presented in this work provide new insights into the cultivation aspects of A. namibiensis and illustrate the challenges on the way to a comprehensive understanding of the complex relationship between productivity, morphology and rheology in filamentous cultivations.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783736973435\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783736973435","offer_id":47437146980677,"sku":"9783736973435","price":44.28,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/6479a5e0-6c41-4f84-92a7-8e0ee5835ed9.jpg?v=1776487497","url":"https:\/\/shop.autorenwelt.de\/products\/production-of-labyrinthopeptin-a1-with-actinomadura-namibiensis-von-sebastian-tesche","provider":"Autorenwelt Shop","version":"1.0","type":"link"}