{"product_id":"polymer-and-quantum-dots-for-immunobased-detection-of-s-typhi-in-water-von-richa-jackeray","title":"POLYMER AND QUANTUM DOTS FOR IMMUNOBASED DETECTION OF S.TYPHI IN WATER","description":"\u003cp\u003eThis thesis is devoted towards the development of  polymeric matrices and bioconjugated quantum dots  for immuno-based detection of S.typhi in water.  Thesis involves the surface modification of nylon  membrane by grafting with GMA using free radical  graft copolymerization. S.typhi specific IgG  immobilized NyM-g-GMA was used as solid phase in  development of ELISA for detection of S.typhi.  Developed sandwich ELISA was found to be specific,  reproducible, reasonably rapid and sensitive with  detection limit of 103cells\/mL. Developed sandwich  ELISA was found to have two-fold higher limit of  detection as compared to the assay performed using  PS plates. For further validation of the developed  immunoassay, water samples were collected from  different sources in and around Delhi and tested for  various parameters of validation. A method for  fluorescence based detection of sandwich  immunocomplex of bacteria and antibody conjugated  CdSe\/ZnS quantum dots on surface modified porous  polycarbonate membrane was also developed.  Fluorescent aggregates were observed on the anti-Sty  IgG immobilized membranes under confocal microscope  and semi-quantified using spectral profiling.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844399295\"\u003e\u003ch3\u003ePolymeric membrane for Ab immobilization and bacteria capturing. Immunocomplex detection by HRP and QDs labeled Ab.\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844399295","offer_id":39471344025693,"sku":"9783844399295","price":79.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/18a758a1-4219-4a65-91bb-1f256bf0b405.jpg?v=1769148248","url":"https:\/\/shop.autorenwelt.de\/products\/polymer-and-quantum-dots-for-immunobased-detection-of-s-typhi-in-water-von-richa-jackeray","provider":"Autorenwelt Shop","version":"1.0","type":"link"}