{"product_id":"phosphorylation-profiling-of-systemin-versus-mamp-signaling-reveals-signaling-specificity-in-plant-immunity-von-rong-li","title":"Phosphorylation profiling of systemin versus MAMP signaling reveals signaling specificity in plant immunity","description":"Plasma membrane-localized pattern recognition receptors (PRRs) detect both microbe-associated molecular patterns (MAMPs) such as flg22 and chitin, and host-derived signals like systemin. While early signaling events (phosphorylation, ROS burst, MAPK activation) are largely similar, they lead to different biological outcomes:\n• MAMPs activate innate immunity,\n• Systemin induces wound and herbivore defense responses.\nTo investigate how specificity arises, phospho-proteomics was applied in tomato cells (wild type vs. systemin receptor-deficient). In total, 4701 phospho-sites on 2119 proteins were identified. Using imputation and clustering, seven temporal phosphorylation patterns were observed:\n• Systemin uniquely caused dephosphorylation at 1–15 min,\n• flg22 triggered an early rapid phosphorylation response,\n• Chitin mainly induced late phosphorylation events.\nSpecificity was illustrated by three protein groups:\n1. RLCK\/PBL kinases → different family members respond in an elicitor-specific way.\n2. S-type anion channel SLAC1 → carries a single regulatory site, differentially phosphorylated depending on the elicitor.\n3. Phosphatase PLL1a → has multiple elicitor-specific phospho-sites (flg22: Ser50–59, chitin: Ser86–89, systemin: Ser163–181).\nNetwork analysis suggested that PLL1a regulates RBOHs, which was functionally validated using phospho-mimic\/dead PLL1 mutants in Nicotiana.\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783689523114\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Buch - 9783689523114","offer_id":55572991246661,"sku":"9783689523114","price":52.88,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/aa3f33f2-7c49-4899-bfbd-4c5e0a818137.jpg?v=1773295822","url":"https:\/\/shop.autorenwelt.de\/products\/phosphorylation-profiling-of-systemin-versus-mamp-signaling-reveals-signaling-specificity-in-plant-immunity-von-rong-li","provider":"Autorenwelt Shop","version":"1.0","type":"link"}