{"product_id":"nucleotide-synthesis-and-regulation-in-a-human-gut-pathogen-von-brendan-burns","title":"Nucleotide synthesis and regulation in a human gut pathogen","description":"\u003cp\u003eUnderstanding the physiology of bacterial pathogens is essential to  design more effective therapies. Thus the biochemical and molecular  characteristics of aspartate carbamoyltransferase, the first step in   pyrimidine biosynthesis, was investigated in the human  pathogen Helicobacter pylori. Using  enzymatic analysis  procedures including NMR spectroscopy and radioactive tracer  analysis, the regulatory properties of H. pylori  ACTase were determined. The results indicated that the first  step in this pathway of H. pylori was highly regulated. To determine  whether ACTase plays an essential role in cell survival in H. pylori,  an attempt was made to construct a mutant deficient in this enzyme  activity. However, no mutants were isolated. These findings suggest  ACTase is essential for survival of H. pylori. As ACTase appeared to  be a potential therapeutic target, the effects of PALA, a potent  inhibitor of this enzyme, was tested on cell viability. However, PALA  had no effect on the viability of the cells. The results suggested H.  pylori transformed the inhibitor into non-cytotoxic products, thus  providing the bacterium with a mechanism of resistance that  appears unique.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838324548\"\u003e\u003ch3\u003eNovel regulation of pyrimidine biosynthesis in the bacterial pathogen Helicobacter pylori\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838324548","offer_id":39498879205469,"sku":"9783838324548","price":79.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/17a050ac-a6e7-40d0-a88c-d6c3a28fa14e.jpg?v=1757741742","url":"https:\/\/shop.autorenwelt.de\/products\/nucleotide-synthesis-and-regulation-in-a-human-gut-pathogen-von-brendan-burns","provider":"Autorenwelt Shop","version":"1.0","type":"link"}