{"product_id":"new-frontiers-in-the-study-of-gene-functions-von-stanley-t-crooke-george-poste-hrsg","title":"New Frontiers in the Study of Gene Functions","description":"\u003cp\u003eThe heat-shock proteins in E. coli are transiently overexpressed af­ ter shift to a higher growth temperature. The genes that encode the HSPs are preceded by promoters transcribed in vitro by a form of RNA poly­ 32 32 merase holoenzyme containing a 32-kd a subunit (Ea ). The a subunit is encoded by the rpoH (htpR) gene, previously identified as a positive 32 effector of the heat-shock response. Our evidence suggests that Ea is the enzyme that transcribes heat-shock genes at all temperatures. The level 32 of a may be regulated at several points: Accumulation of rpoH mRNA 32 is affected by temperature shift, a synthesis is regulated posttranscrip­ 32 tionally, and a is an unstable molecule with a tl\/2 of 5 min. Many mu­ tations in the HSPs are shown to have defects in proteolysis. References Baker. T. A. , Grossman. A. D . . and Gross. C. A. , 1984, A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. A cad. Sci. US. A. 81:6779-6783. Bardwell, J. C. A . . and Craig, E. A . . 1984. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous, Proc. Natl. Acad. Sci. US. A. 81:848-852. Bukhari. A. I. . and Zipser. D . . 1973, Mutants of Escherichia coli with a defect in the degradation of nonsense fragments, Nature New Bioi. 243:238-241. Charette. M. F. , Henderson, G. W. , and Markovitz, A.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9781461290308\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9781461290308","offer_id":39417229115485,"sku":"9781461290308","price":53.49,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/9db4e606-3de6-4fbc-bd0a-be62f7ec6929.jpg?v=1775621024","url":"https:\/\/shop.autorenwelt.de\/products\/new-frontiers-in-the-study-of-gene-functions-von-stanley-t-crooke-george-poste-hrsg","provider":"Autorenwelt Shop","version":"1.0","type":"link"}