{"product_id":"molecular-mechanism-of-fluoroquinolone-resistance-for-m-tb-isolates-von-sulochana-somasundaram-paramasivan-c-n-und-sujatha-n","title":"Molecular mechanism of fluoroquinolone resistance for M.tb isolates","description":"\u003cp\u003eFluoroquinolones are one of the new classes of  antimicrobial agents that are being increasingly  used  against tuberculosis, the most important ones are  ciprofloxacin, ofloxacin, and the new generations  drugs-gatifloxacin and moxifloxacin. The clinical  isolates of Mycobacterium tuberculosis (47 ofloxacin- susceptible and 71 ofloxacin-resistant) strains  obtained from individual patients from various parts  of India were analyzed for gyr A mutation in  quinolone resistant determining region (QRDR). Most  of the mutations were seen clustered in the codons  90, 94 and 95, which is a hot spot region of QRDR.  The types of mutations were correlated with the in  vitro susceptibility pattern of the strains to  ofloxacin. The resistance to fluoroquinolones was  observed predominantly due to gyr A mutations. Gyr A  being a gene with house keeping functions, is highly  conserved in various mycobacterial species. It was  adapted for PCR-RFLP assay using gyr A gene to see  the difference in the profile of 23 standard species  of mycobacteria. M.tuberculosis, M.avium, and  M.intracellulare produced a different profile with  two different restriction enzymes.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844397925\"\u003e\u003ch3\u003ePhenotypic and genotypic resistance to fluoroquinolones for Mycobacterium tuberculosis\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844397925","offer_id":39497198633053,"sku":"9783844397925","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/8820fd4f-08d6-4820-aeb7-dc5d5afa2db7.jpg?v=1757913645","url":"https:\/\/shop.autorenwelt.de\/products\/molecular-mechanism-of-fluoroquinolone-resistance-for-m-tb-isolates-von-sulochana-somasundaram-paramasivan-c-n-und-sujatha-n","provider":"Autorenwelt Shop","version":"1.0","type":"link"}