Molecular Charecterization of Melissococcus pluton of hive honeybees: Control of European foul brood disease with antibiotics and production of antisera and polymerase chain reaction

Molecular Charecterization of Melissococcus pluton of hive honeybees: Control of European foul brood disease with antibiotics and production of antisera and polymerase chain reaction

von Bichitter Sing Rana, Kukkamudi Mohanrao
Taschenbuch - 9783659155550
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Beschreibung

Studied the inlidence of European foul brood disease in relation to temperature and relative humidity in Apis mellifera & A.cerana produced the antiserum against castual bacterium Melissococcus pluton of A.mellifera and detected M.pluton through polymerase chain reaction technique. The molecular cliversity of 8 nucleotides between the isolates was established through ITS (intergenic Transcribed Spacer) primers. Sequenced the 812/550 bps of bacterial isolates and sequences were submitted to the web in European molecular Biology laboratory-European bio informatics Institute (EMBL EBI) Cambridege, UK database and got accession number FN297846 for A.mellifera and FN297845 for A.cerana, M.pluton amplicons. Established phylogenetic relationship of present M.pluton isolates with other reported M.pluton strains with Genbank database. Results revealed that the present isolates were closely related to German stain Melissococca pluton (LTH-3442-AJ). Evaluated 22 antibiotics invitro and ciprofloxacin antibiotic invivo conditions. Detected the ciprofloxacin residues in honey through LSMS chromatography at Indian Institute of Integrative medicines (IIIM) Jammu.

Details

Verlag LAP Lambert Academic Publishing
Ersterscheinung September 2012
Maße 220 mm x 150 mm x 8 mm
Gewicht 219 Gramm
Format Taschenbuch
ISBN-13 9783659155550
Auflage Nicht bekannt
Seiten 136

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