{"product_id":"microbial-production-of-cis-cis-muconic-acid-from-hydrothermally-converted-lignocellulose-von-soren-starck","title":"Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose","description":"Cis,cis-muconic acid receives increasing interest to be produced from renewables. Catabolic microbial pathways can be tailored to accumulate cis,cis-muconic acid from a range of aromatic compounds. A renewable, sustainable and under-valued resource for aromatics is lignin. In this work, using hydrothermal conversion, lignin was depolymerized into hydrolysates with up to 615 mM aromatic monomer content. Catechol-rich hydrolysates were generated for bioconversion with the previously developed cis,cis-muconic acid producers P. putida MA-9 and C. glutamicum MA-2, whereas hydrolysates were guaiacol-rich for Amycolatopsis sp. MA-2. When grown with glucose as a co-substrate, C. glutamicum MA-2 yielded 2.6 g L⁻¹ (100 % yield) cis,cis-muconic acid from catechol. Towards an even more sustainable process, glucose was then replaced by hemicellulose, a non-food renewable. Hemicellulose, a co-constituent of lignin in lignocellulose, was hydrothermally converted into a mixture of C₅ and C₆ sugars. As hemicellulose was mainly converted into xylose (91 % yield), C. glutamicum MA-2 was engineered to utilize this pentose. Fed-batch bioconversion on a catechol-rich Kraft lignin hydrolysate as well as a hemicellulose hydrolysate using C. glutamicum MA-4 yielded 4 g L⁻¹ muconic acids. As the developed process was non-competitive to feed and food, it is a promising starting point for future application in bio-based industrial settings.\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783736975897\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Buch - 9783736975897","offer_id":39938889285725,"sku":"9783736975897","price":36.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/3d751f4a-a3e7-466a-9034-cde155df100d.jpg?v=1780115170","url":"https:\/\/shop.autorenwelt.de\/products\/microbial-production-of-cis-cis-muconic-acid-from-hydrothermally-converted-lignocellulose-von-soren-starck","provider":"Autorenwelt Shop","version":"1.0","type":"link"}