{"product_id":"japanese-quail-erythrocyte-glucose-6-phosphate-dehydrogenase-von-ibrahim-hamdi-shafeeq-yusuf-temel-und-mehmet-ciftci","title":"JAPANESE QUAIL ERYTHROCYTE GLUCOSE 6-PHOSPHATE DEHYDROGENASE","description":"\u003cp\u003eIn this study, glucose 6-phosphate dehydrogenase (G6PD; EC 1.1.1.49) which is an importance enzyme for the carbohydrate metabolism, was purified from quail¿s erythrocyte and characterized. The purification was performed by preparation of hemolysate and 2', 5'-ADP Sepharose-4B affinity chromatography. G6PD from quail¿s erythrocyte was obtained with a yield of 77.17% having a specific activity of 60.40EU\/mg. protein. The overall purification fold was around 4137. The characterization studies were showed: the stable pH value of enzyme to be 7.5 in Tris-HCl buffer, optimum pH value to be 8.0 in Tris-HCl buffer. The optimum temperature was found at 65oC and the optimum ionic strength at 1 M Tris-HCl. Molecular weight of G6PD enzyme from quail¿s erythrocyte was determined as 78.8 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Finally, the KM and Vmax values for substrate (G6P) and coenzyme (NADP+) of the G6PD enzyme were calculated. KM and Vmax values for the NADP+ coenzyme found as 0.001 mM and 0.124 EU\/mL respectively, and the KM and Vmax values for G6P substrate found as 0.012 mM and 0.05 EU\/mL respectively.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786204213224\"\u003e\u003ch3\u003ePURIFICATION AND CHARACTERIZATION OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM JAPANESE QUAIL ERYTHROCYTES\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9786204213224","offer_id":39495983104093,"sku":"9786204213224","price":43.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/31543d15-723a-46f6-b678-fd46d8dc7bf1.jpg?v=1773731113","url":"https:\/\/shop.autorenwelt.de\/products\/japanese-quail-erythrocyte-glucose-6-phosphate-dehydrogenase-von-ibrahim-hamdi-shafeeq-yusuf-temel-und-mehmet-ciftci","provider":"Autorenwelt Shop","version":"1.0","type":"link"}