{"product_id":"in-vitro-regeneration-of-bitter-gourd-from-seedling-explants-von-md-abu-zafur-al-munsur","title":"In vitro regeneration of bitter gourd from seedling explants","description":"\u003cp\u003eA combination of 1.0 mgl-1 2,4-D and 1.0 mgl-1 BAP produced the highest callus frequency in nodal (93.75%), leaf (78.75%) and roots (75.00%) segments. In case of root tips 1.0 mgl-1 2,4-D combined with 0.5 mgl-1 BAP produced the highest callus (72.5%). Nodal segments required minimum number of 8.25 days while root tips required the maximum 32 days for callus induction.  A combination of 2.5 mgl-1 BAP and 0.6 mgl-1 NAA produced the highest callus frequency (86.25%) in nodal segments followed by root segments (85.00%). Among the combinations 1.0 mgl-1 2, 4-D and 1.0 mgl-1 BAP was the most suitable for producing greenish friable callus and 2.0 or 2.5  mgl-1 BAP and  0.30 or 0.60 mgl-1 NAA was suitable for callus induction. A combination of 1.0 mgl-1 2, 4-D and 1.0 mgl-1 BAP exhibited 75.00% and 56.0% shoot direct regeneration from nodal segments and leaf segments, respectively. Nodal segments had 70% shoot regeneration via callus on medium with 2.0 mgl-1 BAP and 0.5 mg L-1 IBA + 0.2 mgl-1 GA3. Leaf segments and root tips had equal percentage (65.0) of shoot regeneration upon culture on medium with 2.0 mgl-1 BAP and 0.2 mgl-1 IAA.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783845438641\"\u003e\u003ch3\u003eRegeneration of bitter gourd\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783845438641","offer_id":39471380398173,"sku":"9783845438641","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/86b450bf-42ee-485e-bc36-89f0b759fde0.jpg?v=1773296827","url":"https:\/\/shop.autorenwelt.de\/products\/in-vitro-regeneration-of-bitter-gourd-from-seedling-explants-von-md-abu-zafur-al-munsur","provider":"Autorenwelt Shop","version":"1.0","type":"link"}