{"product_id":"impact-of-mononuclear-cell-cryopreservation-von-nour-louati","title":"Impact of mononuclear cell cryopreservation","description":"\u003cp\u003eDevelop the technique for separating CMNSP on Ficoll and thawing freezing, then evaluate the impact of -80°C freezing, in the short and long term, on lymphocyte immunophenotyping data.Ten cases of SLPC referred to the CMF unit: 8 cases of SLPC-B (4 cases of CLL and 4 cases of SLPC-B other than CLL) and 1 case of SLPC-T (Sézary syndrome) and 1 case of SLPC with NK cells.Comparison of cell viability before freezing (D1) and after freezing at -80°C (D5 and D30) showed a significant decrease in cell viability after short-term (D5) and long-term (D30) freezing (p\u0026lt;10-3). Comparison of the percentages of expression of the Ac tested and the MFIs between D5 and D30 of freezing at -80°C in DMSO\/SVF5% showed no statistically significant difference. Furthermore, cryopreservation for 5 days and 30 days affected the Matutes score due to a change in the intensity of lambda light chain expression.This technique is simple to implement and could easily be included in research designs for the immunological phenotyping of SLPCs.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786208698263\"\u003e\u003ch3\u003eimmunological phenotype and viability\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9786208698263","offer_id":54253365330245,"sku":"9786208698263","price":43.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/2e76d6e5-4122-44ed-bf9d-7d9699f793d0.jpg?v=1759471696","url":"https:\/\/shop.autorenwelt.de\/products\/impact-of-mononuclear-cell-cryopreservation-von-nour-louati","provider":"Autorenwelt Shop","version":"1.0","type":"link"}