{"product_id":"identification-and-evaluation-of-biomarkers-to-detect-bladder-cancer-von-hafsa-w-abbas","title":"Identification and Evaluation of biomarkers to detect bladder cancer","description":"\u003cp\u003eUrinary Bladder Cancer (UBC) is the 5th most common cancer in the West. Continuous efforts have been made to develop non-invasive urine-based biomarkers with high sensitivity and specificity that would improve patients¿ quality of life, costs and lower the number of cystoscopies. This can then be utilized as part of a multi-biomarker panel to develop a urine test for UBC diagnosis as a single marker is unable to replace current diagnostic invasive tools. Using a (LC-MS\/MS) proteomic approach, 8 UBC and 1 normal bladder cell secretomes were analysed to identify secreted proteins that can be potential candidate biomarkers. phorbol 12-myristate 13-acetate (PMA; PKC activator was used to treat whole secretomes and investigate all proteins released by cells whereas secretomes that were treated with the broad-spectrum inhibitor, Marimastat underwent ultracentrifugation to focus on proteins being shedded. Protein quantification was based upon stable isotope labelling by peptide demethylation. Two methods were utilized to select candidate biomarkers: Human Protein Atlas and Ingenuity Pathway Analysis. EpCAM biology was also examined to see the effects of PMA and Marimastat on its ectodomain.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659589409\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783659589409","offer_id":39439981346909,"sku":"9783659589409","price":55.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/8092b8d2-eada-40a4-8b56-1a01ee180442.jpg?v=1770876165","url":"https:\/\/shop.autorenwelt.de\/products\/identification-and-evaluation-of-biomarkers-to-detect-bladder-cancer-von-hafsa-w-abbas","provider":"Autorenwelt Shop","version":"1.0","type":"link"}