{"product_id":"genetic-transformation-of-sugarcane-method-and-application-von-md-munan-shaik-und-mst-monira-khaton","title":"Genetic Transformation of Sugarcane: Method and Application","description":"\u003cp\u003eTraditional plant breeding techniques, together with  chemical, biotechnological approaches,  have been extensively used to increase crop yields  which are more productive and resistant to  diseases and pathogens. However, conventional  breeding is a lengthy process. Incorporation of  desired gene(s) from foreign source is the prime  needs to overcome the bottleneck. Genetic  transformation of sugarcane deserve the due research  attention for the purpose. Agrobacterium- mediated genetic transformation in differents  sugarcane varieties and regeneration of plantlets  from transformed explants were discussed. The  transformation efficiency in callus, organogenic  pre-treated callus and meristematic spindle leaf  segments at different infection time by five  Agrobacterium strains containing GUS marker gene and  Stress resistance genes (CIPK, CBL and  Gly1) were explained. PCR based confirmation of GUS  reporter gene was done using DNA isolated  from plantlets regenerated from transformed explants.  Phenotypic expression of resistance genes  also explained with the different regeneration medium  containing selective stresses.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844380187\"\u003e\u003ch3\u003eAgrobacterium-mediated Genetic Transformation of Marker and Stress Resistance genes in Saccharum  officinarum L.\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844380187","offer_id":39470066663517,"sku":"9783844380187","price":68.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/c6983714-d41d-4274-ac68-48c19c9dd667.jpg?v=1757656922","url":"https:\/\/shop.autorenwelt.de\/products\/genetic-transformation-of-sugarcane-method-and-application-von-md-munan-shaik-und-mst-monira-khaton","provider":"Autorenwelt Shop","version":"1.0","type":"link"}