{"product_id":"genetic-engineering-of-e-coli-dna-methyltransferase-ecodam-von-hany-elsawy","title":"Genetic engineering of E.coli DNA methyltransferase (EcoDam)","description":"\u003cp\u003eDNA methylation carried out by DNA   methyltransferases (MTases) is widespread in   prokaryotes and eukaryotes. We have changed the   target specificity of EcoDam from GATC to GATT by   directed evolution, combining different random   mutagenesis methods with restriction protection at   GATT sites for selection and screening. After four   generations of mutagenesis   and selection, we obtained enzyme variants with new   specificity for GATT. We conclude that the new   methyltransferases are fully functional   in vivo and in vitro.  The EcoDam and T4Dam DNA-(adenine N6)-  methyltransferases both methylate the adenine   residue in GATC sites. They are highly related in   amino acid sequence and structure but deviate in   their contact to the first base pair of the target   sequence. We have transplanted the T4Dam DNA   recognition module into EcoDam and show that the   EcoDam K9A\/Y138R double mutant is highly active and   specific. This result indicates that there exists a   smooth evolutionary pathway changing the EcoDam DNA   recognition mode to the T4Dam mode without loss of   activity.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838355276\"\u003e\u003ch3\u003eUse of protein engineering to study DNA recognition of E.coli DNA methyltransferase (EcoDam)\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838355276","offer_id":39485422829661,"sku":"9783838355276","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/b131a4ea-33f2-435c-91bb-c16e6929ae3b.jpg?v=1773297633","url":"https:\/\/shop.autorenwelt.de\/products\/genetic-engineering-of-e-coli-dna-methyltransferase-ecodam-von-hany-elsawy","provider":"Autorenwelt Shop","version":"1.0","type":"link"}