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A multiple utility crop needs improvement in order to screen better genotypes with higher seed yield, higher oil content, fast growth, resistance against pest and disease and other performance characteristics. The selection of good seed source and assessment of genetic variation is the prerequisite for the success of any large scale plantation programme. This study was conducted in College of Forestry, Vellanikkara, Trichur, during the period 2005-2007 to evaluate different seed sources Jatropha curcas, a potential source of producing biodiesel for their genetic variation and to standardize efficient clonal propagation techniques. The study involved the evaluation of seed sources for their seed and seedling parameters as well as field performance of the plants.