{"product_id":"engineering-hexuronic-acid-catabolism-synthetic-biology-applications-von-hugo-dantas","title":"Engineering Hexuronic Acid Catabolism","description":"\u003cp\u003eMetabolic engineering targets for the production of high-value compounds. Citrus peel is produced ~15,000,000 t per year worldwide causing environmental problems. Its main constituent is D-galacturonic acid and can be converted to useful chemicals using genetically engineered strains. Engineered Aspergillus niger were used in solid and submerged state fermentation to convert orange peel to L-galactonate in a consolidate process. The gaaB coding for L-galactonate dehydrogenase was deleted (¿gaaB) and ii) the gaaB was deleted and the gaaA coding for D-galacturonate reductase was overexpressed (¿gaaB-gaaA). ¿gaaB was able to convert up to 87 % of D-galacturonic acid to L-galactonate. Also, in this work, the eukaryotic D-Glucuronic acid pathway was studied. A decarboxylase that converts 3-keto-L-gulonate to L-xylulose remains poorly characterized and the gene is not known. A coupled enzyme assay was used to detect its activity. In the assay, ammonium sulfate precipitates from bovine liver extract were coupled with engineered L-gulonate-3-dehydrogenase and L-xylulose reductase. A 3-keto-L-gulonate decarboxylase activity could not be detected.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659580345\"\u003e\u003ch3\u003eSynthetic Biology Applications\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783659580345","offer_id":39448716476509,"sku":"9783659580345","price":39.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/b5508567-8ae2-4ab6-a235-4da6b8b951ed.jpg?v=1757912529","url":"https:\/\/shop.autorenwelt.de\/products\/engineering-hexuronic-acid-catabolism-synthetic-biology-applications-von-hugo-dantas","provider":"Autorenwelt Shop","version":"1.0","type":"link"}