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The best plant growth of onion resulted from poultry manure with bio-fertilizer. The bio-fertilizer resulted an increase in the parameters of onion plant as compared to without bio-fertilizer treatment. The poorest plant growth observed from farmer practices without bio-fertilizer treatment. It was found that combined application of organic manure and chemical fertilizer along with bio-fertilizer improved soil fertility, soil physical and chemical properties and increased crop yields that resulted in sustained and successful crop production. Therefore, it is suggested to make a fertilizer/ manure management strategy which ensures sufficient nutrient supply for high and stable productivity of onion.