{"product_id":"comparative-study-of-ivf-and-parthenogenesis-in-bubaline-oocytes-von-vivek-mishra-und-a-k-misra","title":"Comparative Study of IVF and parthenogenesis in bubaline oocytes","description":"\u003cp\u003eThe objective of this study was to understand the  pitfalls of the poor oocyte cleavage and subsequent  embryo production by assessing the developmental  competence of the oocytes in buffalo using  parthenogenesis as a model which was compared with  IVF (natural activation). Buffalo ovaries were  collected from slaughter house and matured oocytes  were subjected to either IVF (control) or chemical  activation (treatment)[7% ethanol (ET)for 7 min +  2.5 mM 6-Di methyl amino purine (6-DMAP) for 4 hr,  or 7% ET for 7 min + 10 ug\/ml Cycloheximide (CHX)  for 6 hr or 7% ET for 7 min + 2.5 mM 6-DMAP + 10  ug\/ml CHX for 6 hr]. Fertilized and chemically  activated oocytes were cultured in mSOF medium for  8 days to study embryo development.In conclusion,  the results of parthenogenesis, revealed that  buffalo oocytes had better inherent developmental  competence and the poor cleavage and embryo  development following IVF, in our study, may partly  be due to the poor quality of frozen-thawed sperm,  improper sperm capacitation and\/ or fertilization.  Further, high ambient temperature adversely  affected the cleavage and embryo development  following IVF.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844326963\"\u003e\u003ch3\u003eIVF and parthenogenesis: A comparative study\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844326963","offer_id":39495966359645,"sku":"9783844326963","price":59.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/3bfd65e4-5fd2-44b0-b81c-38be649d7d70.jpg?v=1770878408","url":"https:\/\/shop.autorenwelt.de\/products\/comparative-study-of-ivf-and-parthenogenesis-in-bubaline-oocytes-von-vivek-mishra-und-a-k-misra","provider":"Autorenwelt Shop","version":"1.0","type":"link"}