{"product_id":"cloning-and-expression-of-recombinant-fasciola-antigen-von-nahed-hussien","title":"Cloning and expression of recombinant Fasciola antigen¿","description":"\u003cp\u003eFasciolosis is a liver disease caused by Fasciola hepatica or F. gigantica that causes significant economic  loss in cattles, other animal species and man. The appearance of Fasciola  hepatica populations that are resistant to common flukicidal drugs make a  need for the development of anti-liver fluke vaccines. Though we selected  a target Fasciola gene that expresses (rFhp) protein, in which its native  form shows significant induction to lymphoproliferative response of  Peripheral blood mononuclear cells. Then we amplified the gene  through Polymerase chain reaction process using ¿gt11 specific  primers. This gene was cloned through TOPO (TA) cloning  vector in XL10-Gold competent cells, then positive colonies that contain  rTOPO were used for the excision of the gene to be expressed in  expression vectors. ECORI digestions of the  purified plasmids were done to detect the existence of the target insert.  The results showed the excision of Fh¿400 from recombinant PQE32 vector and  its size determined at 500bp. Expression and screening of small cultures  was done and a 6xHis-tagged protein was purified \u0026amp; stained on SDS-PAGE that appeared at about 14.5 kDa.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659147913\"\u003e\u003ch3\u003eNew Hope for Fascioliasis Vaccine\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783659147913","offer_id":39487730483293,"sku":"9783659147913","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/a0a2eadc-baf9-4f28-b770-0e79ae95e3ec.jpg?v=1773295066","url":"https:\/\/shop.autorenwelt.de\/products\/cloning-and-expression-of-recombinant-fasciola-antigen-von-nahed-hussien","provider":"Autorenwelt Shop","version":"1.0","type":"link"}