{"product_id":"characterization-of-lectin-carbohydrate-interactions-von-frederike-eggers","title":"Characterization of Lectin-Carbohydrate interactions","description":"\u003cp\u003eGalectins are a family of endogenous lectins with adhesion growth-regulatory activity binding galactosides. They are also known to play important roles in cancer, inflammation and a lot of other biological processes. Because of such features characterisation of galectin-carbohydrate interactions and identification of binding sites is of crucial importance for drug design. In the present study rat galectin-5 (rGal5) peptides containing key amino acids involved in carbohydrate binding were identified by CREDEX-MS of rGal5 and lactose - in complete agreement with the binding sites of the molecular modelling structure. Until now there are no X-Ray and NMR structures available for galectin-5 therefore studying galectin-5-carbohydrate interaction is very interesting. Both identified carbohydrate-binding peptides were synthesized and their affinity for lactose was demonstrated by affinity-MS.To get a more detailed view on the affinity and interaction kinetics of the CRD peptides and full galectin-5 with lactose SAW-bioaffinity measurements were performed. For real-time study of carbohydrate-lectin interaction a newly developed online-coupling of surface-acoustic wave measurements with ESI-Ion Trap MS was used. With this system the peptides interacting with on chip bound carbohydrate can be directly measured, enabling real time studies of peptide\/protein-carbohydrate interactions.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783639425314\"\u003e\u003ch3\u003eNew mass spectrometry approaches for characterization of Protein-Sugar interactions\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783639425314","offer_id":39467083333725,"sku":"9783639425314","price":32.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/094e38f7-7fac-4c63-818a-3550522ddab8.jpg?v=1773726014","url":"https:\/\/shop.autorenwelt.de\/products\/characterization-of-lectin-carbohydrate-interactions-von-frederike-eggers","provider":"Autorenwelt Shop","version":"1.0","type":"link"}