{"product_id":"carica-papaya-lipase-a-versatile-enantioselectivity-biocatalyst-von-jim-chen-und-shau-wei-tsai","title":"Carica papaya lipase: a versatile enantioselectivity biocatalyst","description":"\u003cp\u003eThe papaya lipase stored in the crude papain from  spay-dried latex of Carica papaya has been  discovered as a versatile enantioselectivity  biocatalyst to obtain chiral acids by using  hydrolytic resolution of their racemic esters. With  adopting the enzyme screening, purification, medium  engineering and substrate engineering tactics  can effectively improve the ability of lipase- catalyzed hydrolysis of (R,S)-naproxen ester. By  employing partially purified lipase or an  appropriate organo-soluble base (ex: Triethylamine) addition in water-saturated isooctane, both  have superior enhancement of lipase activity or  enantioselectivity. In addition, it was also found  that initial rate enhancement for (S)-ester  displayed as increasing the inductive parameter  (electron-withdraw capability), therefore it further  implies the acylaction step must be the rate- limiting step during enzyme-catalyzed process.  Besides, a novel means of applying substrate- assisted catalysis demonstrated the proton shuttle  device acts as an effective tool for improving the  lipase activity by forming an extra intra-molecular  hydrogen bond (11.5~11.9 KJ\/mol) in the transition  state.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844317305\"\u003e\u003ch3\u003eApplications of medium engineering and substrate engineering to improving Carica papaya lipase- catalyzed kinetic resolution of (R,S)-naproxen esters\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844317305","offer_id":39495917273181,"sku":"9783844317305","price":59.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/0cdf7b2b-d5a8-49b1-aecb-58bc05b4701c.jpg?v=1770529561","url":"https:\/\/shop.autorenwelt.de\/products\/carica-papaya-lipase-a-versatile-enantioselectivity-biocatalyst-von-jim-chen-und-shau-wei-tsai","provider":"Autorenwelt Shop","version":"1.0","type":"link"}