{"product_id":"bacterial-flora-in-crohns-disease-tissues-von-gert-de-hertogh","title":"Bacterial flora in Crohn''s disease tissues","description":"\u003cp\u003eCrohn¿s disease is an idiopathic, chronic, segmental  and transmural inflammatory bowel disease. Three  models implying a role for bacteria have been  proposed: infection by a single, hitherto unknown  pathogen; an unbalanced composition of the bowel  flora; and an abnormally increased passage of  bacteria through the bowel wall. We tested these  hypotheses by an experimental approach combining  morphology and molecular bacteriology. The test  protocol consisted of microdissection of Crohn¿s  lesions from histological slides, DNA isolation,  purification and sequencing of bacterial DNA, and  identification of bacteria based on these sequences.  Legionellaceae were specific for Crohn. Ulcers in  Crohn¿s disease were also enriched in  Enterobacteriaceae. The bowel wall in Crohn¿s  disease further contained larger numbers of bacteria  than in controls. Overall, it is not yet possible to  exclude the existence of a unique pathogen, such as  in Helicobacter pylori-induced gastritis. Full-blown  Crohn¿s disease is however clearly associated with  important disturbances of the normal composition and  spatial distribution of the intestinal flora.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838309019\"\u003e\u003ch3\u003eA molecular approach\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838309019","offer_id":39497093611613,"sku":"9783838309019","price":79.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/03f4deb6-1d61-4acf-958e-a645d15a8708.jpg?v=1769148744","url":"https:\/\/shop.autorenwelt.de\/products\/bacterial-flora-in-crohns-disease-tissues-von-gert-de-hertogh","provider":"Autorenwelt Shop","version":"1.0","type":"link"}