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Preemptive analgesia sugests that the application of analgesic in prior to proceeding of noxious stimuli prevent the sensibility of the central nervous system which provokes the pain. The aim of this study was to investigate preemptive analgesic effects of intraperitoneally administrated midazolam in different doses and midazolam with morphine and diclofenac, in rat model.Systemically administered midazolam had preemptive analgesic effects on acute thermal, and acute inflammatory induced nociception in rats. The antinociceptive potency of midazolam enhanced with morphine and diclofenac.