{"product_id":"an-in-vitro-study-of-the-expression-purification-and-intramolecular-interactions-of-hepatoma-up-regulated-protein-hurp-von-eva-ka","title":"An in vitro study of the expression, purification and intramolecular interactions of Hepatoma Up Regulated Protein (HURP)","description":"\u003cp\u003eBachelor Thesis from the year 2011 in the subject Biology - Micro- and Molecular Biology, grade: A+ or 10.0, Democritus University Of Thrace (Molecular Biology and Genetics), language: Modern Greek, abstract: This manuscript is a diploma undergraduate thesis written in Greek; Research was conducted at the University of Thrace, at the Department of Molecular Biology and Genetics. Hepatoma Up Regulated Protein (HURP) is a microtubule-associated protein essential for the assembly of the mitotic spindle. HURP binds microtubules via its N-terminal domain, which is inhibited by HURP¿s C-terminal tail. The kinase Aurora A activates HURP by phosphorylating its C-terminus, which dissociates from the N-terminal microtubule binding domain. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis diploma thesis explores how the autoinhibitory interaction between the N- and C-termini of HURP affect binding to proteins with key functions in spindle assembly (e.g., the mitotic motor Eg5 and the microtubule associated proteins TPX2 andXMAP125). I used molecular cloning techniques to generate recombinant GST- and His tagged chimeras of HURP¿s N- and C-termini. I optimized conditions for the expression of these proteins in bacteria and their purification by liquid chromatography methods using an FPLC system. I performed pull-down assays by incubating the recombinant proteins with HeLa cell extracts and ran western blots to examine the interactions of HURP with Eg5, TPX2 and XMAP125.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783668190436\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783668190436","offer_id":39435317575773,"sku":"9783668190436","price":47.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/2dcc3b6c-d842-4d8a-ae5f-22ee25939963.jpg?v=1778305464","url":"https:\/\/shop.autorenwelt.de\/products\/an-in-vitro-study-of-the-expression-purification-and-intramolecular-interactions-of-hepatoma-up-regulated-protein-hurp-von-eva-ka","provider":"Autorenwelt Shop","version":"1.0","type":"link"}