{"product_id":"amylase-production-through-agro-waste-von-shalini-rai","title":"Amylase Production Through Agro-Waste","description":"\u003cp\u003eIncreased production of ¿-amylase was observed with Bacillus amyloliquefaciens in the present study with wheat flour among the various agro-byproducts tested. The amylase from the bacterium was optimally active at a temperature of 37°C for 72h at pH 4±0.2 by using solid substrate (wheat flour) with the initial moisture of 80% and inoculated with Bacillus amyloliquefaciens of ¿2×106 cfu ml-1 of inoculum size. Supplementation with 0.04M KH2PO4, 1% maltose as a carbon source, tryptone and ammonium chloride as inorganic and organic nitrogen source in the substrate enhanced the enzyme yield considerably. The ¿-amylase from Bacillus amyloliquefaciens was partially purified by ammonium sulphate precipitation and ion exchange chromatography. Purification of amylase enzyme yielding a 2.05 fold purified enzyme with a specific activity of 11214.70 IU\/mg. Characterization of purified enzyme revealed maximum stability on 65¿C temperature at pH 6±0.2. The enzyme was stable against Ca+2, Fe+3, Mn+2 metal ions however Cu+2 showed inhibitory effect. The characteristics of the enzyme qualify it as a good enzyme in biotechnological applications.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659487590\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783659487590","offer_id":39448752980061,"sku":"9783659487590","price":54.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/b6616ef6-a3fb-432d-9ee8-99baf4c50b53.jpg?v=1773121186","url":"https:\/\/shop.autorenwelt.de\/products\/amylase-production-through-agro-waste-von-shalini-rai","provider":"Autorenwelt Shop","version":"1.0","type":"link"}