{"product_id":"type-3-secretion-systems-methods-and-protocols-von-matthew-l-nilles-danielle-l-jessen-condry-hrsg","title":"Type 3 Secretion Systems","description":"\n                                \n                \u003cp\u003e\n                                        This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful \n                    \n                    \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003e\n                                        series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\n                \n                \u003c\/p\u003e\n                                \n                \u003cp\u003e\n                                        \n                    \u003cbr\u003e\n                                         Practical and comprehensive, \n                    \n                    \u003ci\u003eType 3 Secretion Systems: Methods and Protocols\u003c\/i\u003e\n                                         is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.\n                    \n                    \u003cb\u003e\n                                                \n                        \u003cbr\u003e\n                                                 \n                    \n                    \u003c\/b\u003e\n                                    \n                \u003c\/p\u003e\n                            \n            \u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9781493966479\"\u003e\u003ch3\u003eMethods and Protocols\u003c\/h3\u003e\u003c\/div\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9781493982608\"\u003e\u003ch3\u003eMethods and Protocols\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Hardcover - 9781493966479","offer_id":28577398685789,"sku":"9781493966479","price":106.99,"currency_code":"EUR","in_stock":true},{"title":"Softcover - 9781493982608","offer_id":39417542541405,"sku":"9781493982608","price":117.69,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/a1ba550d-943d-4966-9a67-97e1a4c3b4cd.jpg?v=1772342401","url":"https:\/\/shop.autorenwelt.de\/en\/products\/type-3-secretion-systems-methods-and-protocols-von-matthew-l-nilles-danielle-l-jessen-condry-hrsg","provider":"Autorenwelt Shop","version":"1.0","type":"link"}