{"product_id":"single-chain-fragment-variable-scfv-against-breast-cell-line-mcf-7-von-elham-mahgoub","title":"Single Chain Fragment Variable (scFv) Against Breast Cell Line MCF-7","description":"\u003cp\u003eAbstract Recombinant antibody cloning and phage display technologies proved to be very efficient in producing single-chain antibodies (scFv), against antigen of interest which is MCF-7 breast cancer cell line. The main objectives of this study are to construct single chain variable fragment (scFv) towards MCF-7 breast cancer cells and to characterize scFv antibodies that interacts with MCF-7 breast cancer cells line. In the methods: phage display technologies were used to produce single-chain antibodies (scFv) against (MCF-7) breast carcinoma cell line. The starting material was the mouse B cell hybridoma line C3A8, which generates a monoclonal antibody against breast cancer cells (MCF-7). The integrated cloning, screening, and phage display system allowed to obtain a scFv sequences derived from the hybridoma C3A8 cell line. The scFv gene was then cloned into nova-blue host strain for cloning purposes and then into origami DE host strain for further characterization. The purified single-chain antibody was purified using Immobilized Metal Affinity Chromatography (IMAC).the recombinant antibody technology is a rapid approach to next generation of cancer diagnosis and therapy antibody\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783659606120\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783659606120","offer_id":39429023793245,"sku":"9783659606120","price":71.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/5d4b50e0-9fa0-4ee8-8b3c-f8a98ed49e52.jpg?v=1776406685","url":"https:\/\/shop.autorenwelt.de\/en\/products\/single-chain-fragment-variable-scfv-against-breast-cell-line-mcf-7-von-elham-mahgoub","provider":"Autorenwelt Shop","version":"1.0","type":"link"}