{"product_id":"rna-interference-screens-as-a-tool-for-discovering-gene-function-von-despina-siolas","title":"RNA interference Screens as a Tool for Discovering Gene Function","description":"\u003cp\u003eRecent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell''s genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray.  I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin.  This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783843375092\"\u003e\u003ch3\u003efor cancer genetics studies\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783843375092","offer_id":39470100676701,"sku":"9783843375092","price":79.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/475094d5-6100-449c-b691-3a1fe9d2912e.jpg?v=1758876157","url":"https:\/\/shop.autorenwelt.de\/en\/products\/rna-interference-screens-as-a-tool-for-discovering-gene-function-von-despina-siolas","provider":"Autorenwelt Shop","version":"1.0","type":"link"}