{"product_id":"ribozyme-cleavage-von-unknown","title":"Ribozyme Cleavage","description":"\u003cp\u003eInternship Report from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, Free University of Berlin (Institut für Chemie und Biochemie), course: Methodenmodul Nukleinsäuren, language: English, abstract: The hammerhead ribozyme is a catalytic active RNA molecule that catalyzes the cleavage of RNA. This reaction is part of the in vivo self-cleavage mechanism that is observed in the rolling cycle replication mechanism of viroids. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eThe chemically synthesized hammerhead ribozyme that is used in this experiment forms of two RNA strands that harbor the catalytic activity and a substrate RNA strand as shown in figure 1. In the presence of MgCl2, the ribozyme adopts its active conformation and cleaves substrate strand.\u003c\/p\u003e\u003cp\u003eIn order to demonstrate the cleavage, the substrate strand is radioactively labeled with 32P at the 5¿ end by T4 polynucleotide kinase.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783656823445\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783656823445","offer_id":39447038754909,"sku":"9783656823445","price":9.99,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/3fc3ff57-6d76-47ec-8046-169ed9c55df7.jpg?v=1778132282","url":"https:\/\/shop.autorenwelt.de\/en\/products\/ribozyme-cleavage-von-unknown","provider":"Autorenwelt Shop","version":"1.0","type":"link"}