{"product_id":"riboflavin-production-with-ashbya-gossypii-band-6-von-susanne-katharina-schwechheimer","title":"Riboflavin production with Ashbya gossypii (Band 6)","description":"\u003cp\u003eThe fungus Ashbya gossypii is an important industrial producer of riboflavin, i.e. vitamin B2. Here, we developed and then used a highly sophisticated set-up of parallel 13C tracer studies with labeling analysis by GC\/MS, LC\/MS, 1D, and 2D NMR to resolve carbon fluxes and obtain a detailed picture of the underlying metabolism in the overproducing strain A. gossypii B2 during growth and riboflavin production from a complex industrial medium using vegetable oil as carbon source. Glycine was exclusively used as carbon-two ¿ but not carbon-one (C1) ¿ donor of the vitamin¿s pyrimidine ring due to the proven absence of a functional glycine cleavage system. Yeast extract was the main carbon source during growth, while still contributing 8 % overall carbon to riboflavin. Overall carbon flux from rapeseed oil into riboflavin equaled 80 %. Transmembrane formate flux simulations revealed that the C1-supply displayed a severe bottleneck during the initial riboflavin production, which was overcome in later phases of the cultivation by intrinsic formate accumulation. The transiently limiting C1-pool was successfully replenished by time-resolved feeding of formate or serine. This increased the intracellular precursor availability and resulted in a riboflavin titer increase of 45 %. This study is the first that successfully sheds light on carbon fluxes during the growth and riboflavin production phase by use of 13C tracers and a complementary platform of analytical techniques.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783736998810\"\u003e\u003ch3\u003eA 13C high-resolution metabolic network analysis under industrial process conditions\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783736998810","offer_id":39538817466461,"sku":"9783736998810","price":47.3,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/1bcf8a43-f044-4cd6-8481-7e116eb3ea52.jpg?v=1781325423","url":"https:\/\/shop.autorenwelt.de\/en\/products\/riboflavin-production-with-ashbya-gossypii-band-6-von-susanne-katharina-schwechheimer","provider":"Autorenwelt Shop","version":"1.0","type":"link"}