{"product_id":"quantitative-detection-of-e-coli-o157-h7-in-ground-beef-von-assem-abolmaaty-sayedahmed","title":"Quantitative Detection of E.coli O157:H7 in Ground Beef","description":"\u003cp\u003eEscherichia coli O157:H7 is recognized as a major human enteropathogen. A direct spectrophotometric immune agglutination - the first assay had ever published - was developed for quantitation of specific Escherichia coli O157 IgG. A new cell lysis solution designated TZ was also developed and found superior to a variety of cell lysing methods prior to PCR. A 6-fold increase of extracted E. coli 0157:H7 cells from seeded in ground beef was achieved via developing a differential extraction procedure. Highest amplification of E. coli O157: H7 was achieved with the aid of TZ lysis solution, extraction procedures and the optimization of PCR procedure; resulted in the detection of 100 cells of SLT-1 and 50 cells with SLT-2 per 10 grams of ground beef.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786139971831\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9786139971831","offer_id":39464593948765,"sku":"9786139971831","price":76.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/528451a5-6adc-482e-b252-cf56a9e39609.jpg?v=1769151171","url":"https:\/\/shop.autorenwelt.de\/en\/products\/quantitative-detection-of-e-coli-o157-h7-in-ground-beef-von-assem-abolmaaty-sayedahmed","provider":"Autorenwelt Shop","version":"1.0","type":"link"}