{"product_id":"modification-of-ti-plasmid-to-construction-dti-vector-for-gene-cloning-von-hisham-faiadh","title":"Modification of Ti Plasmid to Construction dTi Vector for Gene Cloning","description":"\u003cp\u003eThe extracted DNA of Agrobacterium tumefaciens isolates were subjected to PCR for amplifying 16S rRNA and for amplifying T-DNA fragment then subjected to gel electrophoresis. The individual band of the 16S rRNA gene was characterized by 1479bp and of the T-DNA fragment by 1200bp. The products were comparison with the standard molecular DNA ladder (1200 and 1500bp). The purified ¿-lactamase gene was cut from PGLO plasmid ligated by use T4DNA ligase enzyme with the Ti plasmid which disarmed T-DNA by using the same restriction enzyme. The result was indicated by using the E.coli K12 for carries the Ti plasmid vector which contain a ¿-Lactamase gene when put the antibiotic ampicillin in different consternation into the LB, MHA plate only the colonies which that have picked up exogenous DNA (dTi plasmid DNA ) can grow that is mean it become resistance to ampicillin by using dTi vector.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786134981644\"\u003e\u003ch3\u003e\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9786134981644","offer_id":39446418849885,"sku":"9786134981644","price":49.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/08bff563-2526-4f3b-a4d4-203bb8f7fc91.jpg?v=1773301108","url":"https:\/\/shop.autorenwelt.de\/en\/products\/modification-of-ti-plasmid-to-construction-dti-vector-for-gene-cloning-von-hisham-faiadh","provider":"Autorenwelt Shop","version":"1.0","type":"link"}