{"product_id":"into-the-membrane-and-beyond-von-mirjam-klepsch","title":"Into the Membrane and Beyond","description":"\u003cp\u003eMembrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. Unfortunately, over-expression of membrane proteins in E. coli is usually toxic. I have studied the effects of the expression of membrane proteins, including the human KDEL receptor, on BL21(DE3) and its derivatives. Saturation of the Sec translocon, a cytoplasmic membrane associated protein conducting channel that mediates the insertion\/ biogenesis of membrane proteins, appeared to be the prime reason for the toxicity of membrane protein over-expression.   The obtained data have resulted in many leads and ideas to further improve membrane protein over-expression yields in E. coli, e.g. we have engineered a plasmid (pLemo) that helps avoiding saturation of the Sec translocon upon membrane protein over-expression in E. coli.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783848419692\"\u003e\u003ch3\u003eImproving Membrane Protein Over-Expression in Escherichia coli\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783848419692","offer_id":39491167649885,"sku":"9783848419692","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/d605cdb2-0d2a-4d78-998e-617e5af5016f.jpg?v=1759211933","url":"https:\/\/shop.autorenwelt.de\/en\/products\/into-the-membrane-and-beyond-von-mirjam-klepsch","provider":"Autorenwelt Shop","version":"1.0","type":"link"}