{"product_id":"characterize-mammalian-transcriptome-complexity-von-jiaqian-wu","title":"CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY","description":"\u003cp\u003eIn my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed  to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI\/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783844320602\"\u003e\u003ch3\u003eFROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783844320602","offer_id":39470121025629,"sku":"9783844320602","price":79.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/09468345-5ecf-4448-bb5f-98026a1b8594.jpg?v=1758952808","url":"https:\/\/shop.autorenwelt.de\/en\/products\/characterize-mammalian-transcriptome-complexity-von-jiaqian-wu","provider":"Autorenwelt Shop","version":"1.0","type":"link"}