{"product_id":"characterization-of-the-cd83-promoter-enhancer-complex-in-human-dendritic-cells-von-marcello-stein","title":"Characterization of the CD83 promoter\/enhancer complex","description":"\u003cp\u003eCD83 is one of the best-known surface markers for mature dendritic cells (DC). As CD83 is specifically upregulated upon DC maturation, a highly cell type- and maturation status-specific gene regulation is hypothesized. To elucidate the regulation mechanisms of CD83 expression in human DC, ChIP-chip-microarray-, biocomputational-, reporter, CpG-methylation- and EMSA -analyses were performed. By these studies, a tripartite transcriptional activation complex composed of an upstream regulatory element, a minimal promoter and an enhancer was identified. Their specific arrangement and cooperation in trans, mediated by a complex framework of IRF- and NFkB-binding sites, has not been reported for any other gene so far. The characterization of the CD83 promoter complex not only allows a detailed look into the elaborate mechanisms of the immune response in general and the biology of DC in particular, but also exemplarily unfolds the cooperation of various promoter elements in a highly cell type- and status-specific immune cell related promoter. Thus, for the first time, a human mature DC-specific promoter is described that might become an important tool for transcriptional targeting of DC.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838136684\"\u003e\u003ch3\u003ein human dendritic cells\u003c\/h3\u003e\u003c\/div\u003e","brand":"Libri","offers":[{"title":"Softcover - 9783838136684","offer_id":39441227546717,"sku":"9783838136684","price":79.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/7d0e4bfa-8b77-4a70-98b4-1bb8c68c8ae2.jpg?v=1777957226","url":"https:\/\/shop.autorenwelt.de\/en\/products\/characterization-of-the-cd83-promoter-enhancer-complex-in-human-dendritic-cells-von-marcello-stein","provider":"Autorenwelt Shop","version":"1.0","type":"link"}