{"product_id":"a-pneumococcal-protein-glycocapture-approach-von-alfonso-olaya-abril","title":"A pneumococcal protein glycocapture approach","description":"\u003cp\u003eEmergence of pneumococcal multi-drug resistant clinical isolates prompts the need for a more effective vaccine for the prevention of disease. For this purpose, glycoproteins could be excellent candidates because of their implication in a number of biological processes, such as cell-to-cell recognition and the host immune response. In this study, methods widely used to capture potential surface proteins independently of their glycosylation status were carried out, and Concanavalin A (ConA) and Hydrazide subproteomes were obtained from different pneumococcal subcellular fractions (membrane, lysate, secretome and membrane-derived vesicles (MVs)) followed by protein LC-MS\/MS analysis. In addition, a 2DE multilectinblotting (2DEMLB) analysis, using ConA, WGA, UEA, BC-1 and PNA lectins, was performed. Alfonso Olaya Abril*, Lidia Gómez Gascón, Irene Jiménez Munguía, Michael J. McConnell, Meritxell García Quintanilla, Ignacio Obando Santaella, Manuel José Rodríguez Ortega.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9786202101806\"\u003e\u003ch3\u003eto discover surface proteins by using the reference strain Streptococcus pneumoniae R6\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9786202101806","offer_id":40149668790365,"sku":"9786202101806","price":35.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/3b331b96-1d57-45f3-b930-49d50c61f32a.jpg?v=1773727594","url":"https:\/\/shop.autorenwelt.de\/en\/products\/a-pneumococcal-protein-glycocapture-approach-von-alfonso-olaya-abril","provider":"Autorenwelt Shop","version":"1.0","type":"link"}